3RCP
Crystal structure of the FAPP1 pleckstrin homology domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 31.025, 31.025, 216.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.826 - 1.900 |
R-factor | 0.2328 |
Rwork | 0.230 |
R-free | 0.25530 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.573 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | HKL2Map |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.712 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.517 | |
Number of reflections | 15364 | |
<I/σ(I)> | 6.7 | |
Completeness [%] | 96.4 | 98.1 |
Redundancy | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | precipitant solution containing 0.1 M NaAc, pH 4.6, 50mM (NH4)2SO4 and 15% PEG1000., VAPOR DIFFUSION, HANGING DROP, temperature 291K |