3RCN
Crystal Structure of Beta-N-Acetylhexosaminidase from Arthrobacter aurescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 74.003, 74.003, 409.212 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.979 - 2.514 |
| R-factor | 0.176 |
| Rwork | 0.170 |
| R-free | 0.23100 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.221 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 24024 | |
| <I/σ(I)> | 7.4 | 6.77 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.2 | 18.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M Bis-Tris Propane pH 7.0, 1.5 M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
