3RC8
Human Mitochondrial Helicase Suv3 in Complex with Short RNA Fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-18 |
| Detector | MAR300 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 94.430, 94.430, 88.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.1803 |
| Rwork | 0.177 |
| R-free | 0.23780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rc3 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.660 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.230 | 2.900 |
| Rmerge | 0.075 | 0.039 | 0.407 |
| Number of reflections | 19411 | ||
| <I/σ(I)> | 11.6 | 35.15 | 4.46 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 5.6 | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 6% PEG 3350, 0.1 M Na citrate, 10 % glycerol, pH 6.5, vapor diffusion, hanging drop, temperature 277K |
