3RBY

Crystal structure of uncharacterized protein YLR301w from Saccharomycces cerevisiae

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-15
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97939
Spacegroup name	P 65 2 2
Unit cell lengths	122.320, 122.320, 174.001
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.240 - 2.301
R-factor	0.2064
Rwork	0.204
R-free	0.24730
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.199
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.085	0.314
Number of reflections	34825
<I/σ(I)>	27.8	3.3
Completeness [%]	97.9	97.4
Redundancy	8.7	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	295	33% PEGMME 550, 0.1M HEPES, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
1	VAPOR DIFFUSION, HANGING DROP	7.3	295	33% PEGMME 550, 0.1M HEPES, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K