3RBX
MthK RCK domain D184N mutant, Ca2+-bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 119.120, 119.120, 351.052 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.488 - 2.800 |
R-factor | 0.2417 |
Rwork | 0.240 |
R-free | 0.27390 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.054 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.500 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 37176 | |
<I/σ(I)> | 13.6 | |
Completeness [%] | 96.5 | 96.5 |
Redundancy | 11.3 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 295 | 20% PEG 3350, 0.1 M CaCl2, 0.1 M MES, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K, pH 5.7 |