3RBL
Crystal structure of Human aromatic L-amino acid decarboxylase (AADC) in the apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 179.640, 179.640, 74.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.240 |
| R-factor | 0.239 |
| Rwork | 0.236 |
| R-free | 0.29430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rbf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.271 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 127.025 | 49.823 | 3.410 |
| High resolution limit [Å] | 3.240 | 10.230 | 3.240 |
| Rmerge | 0.038 | 0.402 | |
| Total number of observations | 3939 | 17828 | |
| Number of reflections | 20098 | ||
| <I/σ(I)> | 13.8 | 14.8 | 1.9 |
| Completeness [%] | 99.8 | 97.7 | 100 |
| Redundancy | 6.4 | 5.5 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapor diffusion followed by soaking | 7 | 294 | crystals obtained from holoAADC in 0.1 M HEPES pH 7.0, 28-30% Jeffamine ED 2001 pH 7.0 were transferred in a ML droplet to yield apoAADC, vapor diffusion followed by soaking, temperature 294K |
| 1 | vapor diffusion followed by soaking | 7 | 294 | crystals obtained from holoAADC in 0.1 M HEPES pH 7.0, 28-30% Jeffamine ED 2001 pH 7.0 were transferred in a ML droplet to yield apoAADC, vapor diffusion followed by soaking, temperature 294K |
| 1 | vapor diffusion followed by soaking | 7 | 294 | crystals obtained from holoAADC in 0.1 M HEPES pH 7.0, 28-30% Jeffamine ED 2001 pH 7.0 were transferred in a ML droplet to yield apoAADC, vapor diffusion followed by soaking, temperature 294K |
| 1 | vapor diffusion followed by soaking | 7 | 294 | crystals obtained from holoAADC in 0.1 M HEPES pH 7.0, 28-30% Jeffamine ED 2001 pH 7.0 were transferred in a ML droplet to yield apoAADC, vapor diffusion followed by soaking, temperature 294K |
| 1 | vapor diffusion followed by soaking | 7 | 294 | crystals obtained from holoAADC in 0.1 M HEPES pH 7.0, 28-30% Jeffamine ED 2001 pH 7.0 were transferred in a ML droplet to yield apoAADC, vapor diffusion followed by soaking, temperature 294K |
| 1 | vapor diffusion followed by soaking | 7 | 294 | crystals obtained from holoAADC in 0.1 M HEPES pH 7.0, 28-30% Jeffamine ED 2001 pH 7.0 were transferred in a ML droplet to yield apoAADC, vapor diffusion followed by soaking, temperature 294K |
| 1 | vapor diffusion followed by soaking | 7 | 294 | crystals obtained from holoAADC in 0.1 M HEPES pH 7.0, 28-30% Jeffamine ED 2001 pH 7.0 were transferred in a ML droplet to yield apoAADC, vapor diffusion followed by soaking, temperature 294K |
| 1 | vapor diffusion followed by soaking | 7 | 294 | crystals obtained from holoAADC in 0.1 M HEPES pH 7.0, 28-30% Jeffamine ED 2001 pH 7.0 were transferred in a ML droplet to yield apoAADC, vapor diffusion followed by soaking, temperature 294K |
| 1 | vapor diffusion followed by soaking | 7 | 294 | crystals obtained from holoAADC in 0.1 M HEPES pH 7.0, 28-30% Jeffamine ED 2001 pH 7.0 were transferred in a ML droplet to yield apoAADC, vapor diffusion followed by soaking, temperature 294K |
| 1 | vapor diffusion followed by soaking | 7 | 294 | crystals obtained from holoAADC in 0.1 M HEPES pH 7.0, 28-30% Jeffamine ED 2001 pH 7.0 were transferred in a ML droplet to yield apoAADC, vapor diffusion followed by soaking, temperature 294K |
