3RBJ
Crystal Structure of the lid-mutant of Streptococcus agalactiae Sortase C1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-23 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.477, 49.418, 52.708 |
| Unit cell angles | 87.86, 70.47, 89.40 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.24983 |
| Rwork | 0.247 |
| R-free | 0.30360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.732 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.550 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.052 | 0.402 |
| Number of reflections | 17654 | |
| <I/σ(I)> | 12.2 | 2.9 |
| Completeness [%] | 96.3 | 93.9 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.44 | 277 | 10% PEG2000 MME, 100 mM MES, pH 6.44, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
