3RBJ
Crystal Structure of the lid-mutant of Streptococcus agalactiae Sortase C1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-02-23 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 |
Unit cell lengths | 43.477, 49.418, 52.708 |
Unit cell angles | 87.86, 70.47, 89.40 |
Refinement procedure
Resolution | 25.000 - 2.300 |
R-factor | 0.24983 |
Rwork | 0.247 |
R-free | 0.30360 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.732 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.550 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.052 | 0.402 |
Number of reflections | 17654 | |
<I/σ(I)> | 12.2 | 2.9 |
Completeness [%] | 96.3 | 93.9 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.44 | 277 | 10% PEG2000 MME, 100 mM MES, pH 6.44, VAPOR DIFFUSION, HANGING DROP, temperature 277K |