3RB9

Crystal structure of the M. tuberculosis beta clamp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	71.903, 233.247, 124.301
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.810 - 3.000
R-factor	0.211
Rwork	0.209
R-free	0.25030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.424
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	41.100
High resolution limit [Å]	3.000
Number of reflections	21391
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	9% Isopropanol, 12% PEG 4000, 0.1M Na HEPES , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K