3RA8

Structural studies of AAV8 capsid transitions associated with endosomal trafficking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	113
Detector technology	IMAGE PLATE
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.00
Spacegroup name	P 63 2 2
Unit cell lengths	257.940, 257.940, 448.690
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.700
R-factor	0.246
Rwork	0.245
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	185894
Completeness [%]	77.9	57.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	20 mM Tris pH 7.5, 4% PEG 8000, 1M NaCl. Crystals presoaked in pH 5.5 cryosolvent for 24 hours before data collection, VAPOR DIFFUSION, HANGING DROP, temperature 293K