3RA0
Crystal Structure of a StWhy2 K67A-dT32 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.08 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 166.465, 166.465, 166.465 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.427 - 2.451 |
R-factor | 0.2514 |
Rwork | 0.250 |
R-free | 0.26730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 3N1J |
RMSD bond length | 0.003 |
RMSD bond angle | 0.705 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.450 |
Number of reflections | 7685 |
<I/σ(I)> | 24.5 |
Completeness [%] | 99.7 |
Redundancy | 17.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 100mM Tris pH8.0, 15% PEG6000, 1200mM LiCl, vapor diffusion, hanging drop, temperature 298K |