3R9W

Crystal structure of Era in complex with MgGDPNP and nucleotides 1506-1542 of 16S ribosomal RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	88.946, 85.604, 67.184
Unit cell angles	90.00, 111.20, 90.00

Refinement procedure

Resolution	25.913 - 2.050
R-factor	0.1908
Rwork	0.188
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iev
RMSD bond length	0.008
RMSD bond angle	1.167
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.120
High resolution limit [Å]	2.050	4.410	2.050
Rmerge	0.078	0.054	0.665
Number of reflections	27524
<I/σ(I)>	14.4	25.62	2.18
Completeness [%]	93.7	99.2	72.9
Redundancy	5.5	6.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	292	0.2M calcium acetate, 40% (v/v) MPD, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 292K