3R9R
Structure of a Phosphoribosylaminoimidazole-succinocarboxamide synthase from Mycobacterium abscessus ATCC 19977 / DSM 44196
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.901, 64.786, 48.357 |
Unit cell angles | 90.00, 110.48, 90.00 |
Refinement procedure
Resolution | 45.300 - 1.849 |
R-factor | 0.1773 |
Rwork | 0.174 |
R-free | 0.23310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1obg |
RMSD bond length | 0.011 |
RMSD bond angle | 1.391 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.880 |
High resolution limit [Å] | 1.849 | 5.020 | 1.849 |
Rmerge | 0.082 | 0.032 | 0.369 |
Number of reflections | 23760 | ||
<I/σ(I)> | 10.1 | ||
Completeness [%] | 99.9 | 99 | 100 |
Redundancy | 3.1 | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 20% PEG3350, Cryo 25% Ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 289K |