3R9M

Crystal structure of the Brox Bro1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-06
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 63
Unit cell lengths	189.204, 189.204, 49.523
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.301 - 1.950
R-factor	0.1494
Rwork	0.149
R-free	0.16710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oew
RMSD bond length	0.012
RMSD bond angle	1.402
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.980
High resolution limit [Å]	1.950	5.290	1.950
Number of reflections	74479
Completeness [%]	99.8	98	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1M MES, 1M sodium formate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K