3R9I

2.6A resolution structure of MinD complexed with MinE (12-31) peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-02-14
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1
Unit cell lengths	64.293, 71.796, 76.644
Unit cell angles	102.64, 95.87, 111.72

Refinement procedure

Resolution	43.417 - 2.600
R-factor	0.2033
Rwork	0.201
R-free	0.24260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q9l
RMSD bond length	0.011
RMSD bond angle	1.356
Data scaling software	SCALA (CCP4_3.3.16)
Phasing software	MOLREP
Refinement software	PHENIX (dev_661)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	73.240	73.240	2.740
High resolution limit [Å]	2.600	8.220	2.600
Rmerge	0.153	0.050	0.570
Total number of observations		4134	18794
Number of reflections	36883
<I/σ(I)>	7.149	17.29	2.37
Completeness [%]	98.5	97.52	98.22
Redundancy	3.5	3.57	3.47

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4	293	20% w/v PEG3350, 100 mM citrate, 200 mM sodium citrate, pH 4.0, VAPOR DIFFUSION, temperature 293K