3R8W
Structure of 3-isopropylmalate dehydrogenase isoform 2 from Arabidopsis thaliana at 2.2 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.812, 211.016, 76.903 |
Unit cell angles | 90.00, 90.15, 90.00 |
Refinement procedure
Resolution | 36.998 - 2.250 |
R-factor | 0.177 |
Rwork | 0.175 |
R-free | 0.20750 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.980 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.000 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 114991 | |
Completeness [%] | 99.7 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.68 | 298 | 20% PEG 4000, 20% glycerol, 0.16M ammonium sulfate, 0.08 sodium acetate trihydrate , pH 4.68, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |