3R89
Crystal structure of orotidine 5-phosphate decarboxylase from Anaerococcus prevotii DSM 20548
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.507, 108.848, 138.171 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.500 - 1.844 |
| R-factor | 0.1604 |
| Rwork | 0.158 |
| R-free | 0.19700 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.388 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.503 | 1.880 |
| High resolution limit [Å] | 1.844 | 1.844 |
| Rmerge | 0.114 | 0.774 |
| Number of reflections | 49114 | |
| <I/σ(I)> | 30.7 | 2.72 |
| Completeness [%] | 94.2 | 92.9 |
| Redundancy | 7.7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 0.2M NaCl, 0.1 M Tris pH 7.0, 30% (w/v) PEG-3000, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
