3R87
Crystal Structure of Orf6 protein from Photobacterium profundum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2008-06-25 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.0332, 0.9329 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 103.370, 103.370, 64.710 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.440 - 1.050 |
R-factor | 0.14921 |
Rwork | 0.149 |
R-free | 0.16481 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.028 |
RMSD bond angle | 2.162 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.440 | 1.077 |
High resolution limit [Å] | 1.050 | 1.050 |
Number of reflections | 88811 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES monohydrate, 1.6M Magnesium sulfate heptahydrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |