3R6P
Crystal structure of abscisic acid-bound PYL10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Collection date | 2011-03-16 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 68.327, 68.327, 63.396 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.700 |
R-factor | 0.2428 |
Rwork | 0.239 |
R-free | 0.27770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3uqh |
RMSD bond length | 0.012 |
RMSD bond angle | 1.411 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.700 |
Number of reflections | 4929 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 20% PEG monoethyl ether 5000, 0.1M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |