3R6A
Crystal structure of an uncharacterized protein (hypothetical protein MM_3218) from Methanosarcina mazei.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9790 |
Spacegroup name | P 61 |
Unit cell lengths | 62.433, 62.433, 149.595 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.820 - 1.760 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.23400 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.880 | 1.810 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.104 | 0.588 |
Number of reflections | 32561 | |
<I/σ(I)> | 12.8 | |
Completeness [%] | 99.4 | 99.3 |
Redundancy | 22.5 | 21.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 2.1M ammonium sulfate; 0.1M Na acetate; Trehalose dihydrate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |