3R69
Molecular analysis of the interaction of the HDL-receptor SR-BI with the PDZ3 domain of its adaptor protein PDZK1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-10 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.638, 61.396, 64.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.930 - 1.499 |
R-factor | 0.2016 |
Rwork | 0.200 |
R-free | 0.22549 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3r68 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.718 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.499 | 1.499 |
Number of reflections | 29138 | |
Completeness [%] | 94.5 | 66.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 291 | 0.1 M citric acid pH 3.5, 25% PEG 3350, 5% ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 291K |