3R65
MthK channel pore E92Q mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9790 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 63.560, 63.560, 43.973 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.430 - 1.800 |
R-factor | 0.20601 |
Rwork | 0.205 |
R-free | 0.22520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ldc |
RMSD bond length | 0.009 |
RMSD bond angle | 1.087 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.430 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 7959 | |
<I/σ(I)> | 29.3 | 1.5 |
Completeness [%] | 90.2 | 74.8 |
Redundancy | 9.6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 3.0-3.5 M 1,6-Hexandiol, 100 mM MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |