3R63
Structure of ERK2 (SPE) mutant (S246E)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.770, 69.999, 60.117 |
Unit cell angles | 90.00, 109.04, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.700 |
R-factor | 0.19107 |
Rwork | 0.188 |
R-free | 0.24473 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.684 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.700 |
Number of reflections | 40979 |
Completeness [%] | 97.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100mM Bis-Tris buffer, 61mM ammonium Sulfate, 15% PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |