3R4V

Structure of the phage tubulin PhuZ-GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.115889
Spacegroup name	P 21 21 21
Unit cell lengths	47.065, 75.935, 92.798
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.399 - 1.670
R-factor	0.1881
Rwork	0.186
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SeMet model solved using MAD
RMSD bond length	0.007
RMSD bond angle	1.064
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	58.768
High resolution limit [Å]	1.433
Rmerge	0.108
Number of reflections	45554
<I/σ(I)>	6.8
Completeness [%]	82.9
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	15% PEG 6000, 0.1M HEPES, 0.5M Ammonium Acetate, 0.05M MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K