3R3T
Crystal Structure of 30S Ribosomal Protein S from Bacillus anthracis
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 82.680, 82.680, 59.165 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.609 - 2.302 |
| R-factor | 0.225 |
| Rwork | 0.208 |
| R-free | 0.23200 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.384 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_690)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 10636 | |
| <I/σ(I)> | 10.3 | 4.65 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 12.9 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 50 mM magnesium chloride, 0.1 M HEPES, 30 % v/v polyethylene glycol monomethyl ether 550, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
