3R3K
Crystal structure of a parallel 6-helix coiled coil
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2010-05-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.7 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 31.660, 54.480, 128.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.210 - 2.201 |
R-factor | 0.2153 |
Rwork | 0.212 |
R-free | 0.27150 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.285 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.140 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.109 | 0.339 |
Number of reflections | 5716 | |
<I/σ(I)> | 9.3 | 2.2 |
Completeness [%] | 95.6 | 72.1 |
Redundancy | 5.7 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 20 mM sodium L-glutamate, 20 mM alanine (racemic), 20 mM glycine, 20 mM lysine hydrochloride (racemic), 20 mM serine (racemic), 50 mM sodium HEPES, 50 mM MOPS (acid) pH 7.5, 20 % ethylene glycol,10 % PEG 8K, VAPOR DIFFUSION, SITTING DROP, temperature 291K |