3R2K
1.55A resolution structure of As-Isolated FtnA from Pseudomonas aeruginosa (pH 7.5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 172.590, 172.590, 172.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.167 - 1.550 |
| R-factor | 0.1817 |
| Rwork | 0.180 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r2h |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.221 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_625) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.058 | 0.724 |
| Number of reflections | 31980 | |
| <I/σ(I)> | 42.885 | 2.717 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 14.6 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 20% (v/v) 1,4-butanediol, 100 mM HEPES, 200 mM NaCl, pH 7.5, vapor diffusion, temperature 293K |
