3R2D
Crystal Structure of Antitermination Factors NusB and NusE in complex with dsRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-12 |
Detector | MAR 225 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.786, 79.940, 106.599 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.642 - 2.199 |
R-factor | 0.2033 |
Rwork | 0.201 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3d3b |
RMSD bond length | 0.004 |
RMSD bond angle | 0.723 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.1) |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
High resolution limit [Å] | 2.199 | 4.740 | 2.200 |
Rmerge | 0.057 | 0.033 | 0.542 |
Number of reflections | 32257 | ||
<I/σ(I)> | 14.5 | ||
Completeness [%] | 94.7 | 87.9 | 94.5 |
Redundancy | 5.7 | 5.5 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M diammonium phosphate, 20% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |