3R2C
Crystal Structure of Antitermination Factors NusB and NusE in complex with BoxA RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-25 |
| Detector | MAR 225 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.530, 113.008, 65.763 |
| Unit cell angles | 90.00, 102.87, 90.00 |
Refinement procedure
| Resolution | 35.663 - 1.902 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.20180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r2d |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.028 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.102 | 0.047 | 0.520 |
| Number of reflections | 42602 | ||
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 94.2 | 99.5 | 74.5 |
| Redundancy | 5 | 5.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 310 | 0.1 M sodium acetate, 25% PEG8000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
