3R27
Crystal structure of the first RRM domain of heterogeneous nuclear ribonucleoprotein L (HnRNP L)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-06-06 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 |
Unit cell lengths | 43.170, 43.170, 78.530 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.250 - 2.040 |
R-factor | 0.20682 |
Rwork | 0.204 |
R-free | 0.25473 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wex |
RMSD bond length | 0.009 |
RMSD bond angle | 1.112 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.570 | 2.140 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.071 | 0.341 |
Number of reflections | 10541 | |
<I/σ(I)> | 14 | 3.7 |
Completeness [%] | 99.6 | 97.6 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 0.1M HEPES, 25%w/v Polyethylene glycol 3,350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K |