3R1V
Odorant Binding Protein 7 from Anopheles gambiae with Four Disulfide Bridges, in complex with an azo compound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.760, 41.970, 57.940 |
| Unit cell angles | 90.00, 105.97, 90.00 |
Refinement procedure
| Resolution | 31.270 - 2.190 |
| R-factor | 0.2431 |
| Rwork | 0.242 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.270 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 2.250 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.078 | 0.600 |
| Number of reflections | 11865 | |
| <I/σ(I)> | 12.2 | 2.4 |
| Completeness [%] | 99.0 | 99.1 |
| Redundancy | 4.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.75 | 298 | 33 mM HEPES, pH 7.25-8.25, 0.4-1.0 M KCl, 6.6 mM sodium borate, 0.83 M sodium citrate tribasic, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
