3R1O
Odorant Binding Protein 7 from Anopheles gambiae with Four Disulfide Bridges
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-01 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.704, 46.469, 57.438 |
Unit cell angles | 90.00, 103.78, 90.00 |
Refinement procedure
Resolution | 55.790 - 2.100 |
R-factor | 0.2735 |
Rwork | 0.272 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2erb |
RMSD bond length | 0.009 |
RMSD bond angle | 1.250 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.800 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.091 | 0.193 |
Number of reflections | 14055 | |
<I/σ(I)> | 22.1 | 8.8 |
Completeness [%] | 99.8 | 99.3 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.75 | 298 | 33 mM HEPES, pH 7.25-8.25, 0.4-1.0 M KCl, 6.6 mM sodium borate, 0.83 M sodium citrate tribasic, VAPOR DIFFUSION, SITTING DROP, temperature 298K |