3R1M
Structure of bifunctional fructose 1,6-bisphosphate aldolase/phosphatase (aldolase form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 112.543, 112.543, 153.608 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.220 - 1.500 |
| R-factor | 0.19708 |
| Rwork | 0.196 |
| R-free | 0.21461 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1umg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.431 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 78613 | |
| <I/σ(I)> | 51.5 | 5.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.6 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 10% PEG 20000, 0.1M bicine pH 9.0, 2% dioxane, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
