3R1C
Crystal structure of GCGGCGGC duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.92001 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.697, 76.891, 85.398 |
| Unit cell angles | 89.98, 88.61, 77.29 |
Refinement procedure
| Resolution | 19.356 - 2.053 |
| R-factor | 0.2177 |
| Rwork | 0.216 |
| R-free | 0.25710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r1e |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.145 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 60328 | |
| <I/σ(I)> | 17 | 2.8 |
| Completeness [%] | 98.4 | 97.5 |
| Redundancy | 4.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 10 mM MgCl2, cacodylate, 1.0 M Li2SO4, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
