3R15
Structure Treponema Denticola Factor H Binding Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 170 |
| Detector technology | CCD |
| Collection date | 2010-03-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 46.988, 46.988, 168.948 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.079 - 1.701 |
| R-factor | 0.2128 |
| Rwork | 0.210 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qz0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.984 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 39492 |
| Completeness [%] | 99.6 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M HEPES, 1.2 M sodium citrate tribasic dihydrate, 0.2 M sodium thiocyanate, 2.5% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
