3R0R
The 2.3 A structure of porcine circovirus 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-02-11 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.88 |
Spacegroup name | P 1 |
Unit cell lengths | 193.612, 202.163, 230.975 |
Unit cell angles | 90.01, 89.33, 90.07 |
Refinement procedure
Resolution | 39.960 - 2.350 |
Rwork | 0.225 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | GLRF |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 1002451 | |
Completeness [%] | 68.7 | 44.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 6% PEG 3350, 2.5% isopropanol, 0.3 M monium Citrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |