3R0P
Crystal structure of L-PSP putative endoribonuclease from uncultured organism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.414, 82.680, 99.311 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.532 - 1.900 |
| R-factor | 0.1786 |
| Rwork | 0.177 |
| R-free | 0.21240 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.056 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.487 |
| Number of reflections | 42525 | |
| <I/σ(I)> | 29.1 | 2.8 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 8.8 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M Hepes, 0.2M Ammonium sulphate, 25% PEG3350, 1/75 subtilisin, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
