3R0K
Crystal structure of NYSGRC enolase target 200555, a putative dipeptide epimerase from Francisella philomiragia : Tartrate bound, no Mg
Replaces: 3PX5Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 98 |
Detector technology | CCD |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97905 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 121.000, 121.000, 148.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.103 - 2.000 |
R-factor | 0.1668 |
Rwork | 0.165 |
R-free | 0.19690 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.176 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 75038 | |
<I/σ(I)> | 27.6 | 11.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 28.8 | 27.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 294 | Protein (10 mM HEPES pH 7.5, 150 mM Nacl, 10% glycerol, 5 mM DTT; Reservoir (2M Ammonium Sulfate, 100 mM NaCitrate, 200 mM KNaTartrate), Soak (2.4 M Ammonium sulfate, 100 mM NaCitrate pH 5.6, KNaTartrate, 20% glycerol, 10 min, vapor diffusion, sitting drop, temperature 294K, VAPOR DIFFUSION, SITTING DROP |