3R06
Crystal structure of anti-mouse CD3epsilon antibody 2C11 Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Detector technology | ADSC QUANTUM 315 |
Collection date | 2006-06-19 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 106.171, 72.386, 127.487 |
Unit cell angles | 90.00, 109.52, 90.00 |
Refinement procedure
Resolution | 36.020 - 2.500 |
R-factor | 0.193 |
Rwork | 0.191 |
R-free | 0.23600 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.220 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 62077 | |
Completeness [%] | 98.6 | 90.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 298 | 0.1M HEPES, PEG 4000, pH 7.5, EVAPORATION, temperature 298K |