3QZC
Structure of the periplasmic stress response protein CpxP
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-06 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97945, 0.97961, 0.97754 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 88.031, 88.031, 134.931 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.015 - 2.850 |
| R-factor | 0.2494 |
| Rwork | 0.247 |
| R-free | 0.29800 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.183 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.6.3_473) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 6.140 | 2.850 |
| Rmerge | 0.066 | 0.025 | 0.529 |
| Number of reflections | 7733 | ||
| <I/σ(I)> | 16 | ||
| Completeness [%] | 99.8 | 100 | 100 |
| Redundancy | 9.9 | 11.5 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 294 | 9% (v/v) 2-propanol, 0.1 M cacodylate pH 5.6, 0.7 M Zn(OAc)2), vapor diffusion, hanging drop, temperature 294K |
