3QZ0
Structure of Treponema denticola Factor H Binding protein (FhbB), selenomethionine derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.2822 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 46.921, 46.921, 168.801 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.207 - 1.770 |
R-factor | 0.1906 |
Rwork | 0.189 |
R-free | 0.20570 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.038 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE (within PHENIX) |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.770 |
Number of reflections | 19167 |
Completeness [%] | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 1.39 M sodium citrate, 0.1 M HEPES, 0.2 M sodium thiocyanate, 10 mM dithiothreitol, 10% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |