3QYN
Structure of p63 DNA Binding Domain in Complex with a 22 Base Pair A/T Rich Response Element Containing 2 Base Pair Spacer Between Half Sites
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-03 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 63 |
Unit cell lengths | 140.984, 140.984, 119.346 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 61.048 - 2.500 |
R-factor | 0.2121 |
Rwork | 0.210 |
R-free | 0.24540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qym |
RMSD bond length | 0.011 |
RMSD bond angle | 1.635 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.346 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 46625 | |
<I/σ(I)> | 20.1 | 3.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 12.8 | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 20% PEG3350, 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |