3QYM
Structure of p63 DNA Binding Domain in Complex with a 10 Base Pair A/T Rich Response Element Half Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.872, 180.198, 104.379 |
| Unit cell angles | 90.00, 92.62, 90.00 |
Refinement procedure
| Resolution | 19.719 - 3.200 |
| R-factor | 0.2463 |
| Rwork | 0.245 |
| R-free | 0.27090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ady |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.372 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.257 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 36604 | |
| <I/σ(I)> | 5.8 | 1.7 |
| Completeness [%] | 97.1 | 94.5 |
| Redundancy | 3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 10% PEG3350, 0.2 M ammonium formate, 0.1 M Bis-Tris, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
