3QYF
Crystal structure of the CRISPR-associated protein SSO1393 from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97912 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.614, 81.554, 70.155 |
| Unit cell angles | 90.00, 108.30, 90.00 |
Refinement procedure
| Resolution | 28.349 - 1.900 |
| R-factor | 0.1701 |
| Rwork | 0.168 |
| R-free | 0.20700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.981 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.630 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.480 |
| Number of reflections | 50502 | |
| <I/σ(I)> | 14.3 | 3.9 |
| Completeness [%] | 99.2 | 98.6 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 298 | 0.1M Tris pH 8.2, 0.2M CaCl2, 30%PEG400, 4%MPD, 1/70 subtlisin, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
