3QYE

Crystal Structure of Human TBC1D1 RabGAP domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-12
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0809
Spacegroup name	P 43 21 2
Unit cell lengths	117.360, 117.360, 141.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
Rwork	0.199
R-free	0.23900
Structure solution method	MAD
RMSD bond angle	0.011
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	50676
Completeness [%]		100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	2.0-3.2 M ammonium formate, 0.1 HEPES pH 7.5 and 20-25% ethylene glycol, VAPOR DIFFUSION, temperature 298 KK
1	VAPOR DIFFUSION	7.5	298	2.0-3.2 M ammonium formate, 0.1 HEPES pH 7.5 and 20-25% ethylene glycol, VAPOR DIFFUSION, temperature 298 KK
1	VAPOR DIFFUSION	7.5	298	2.0-3.2 M ammonium formate, 0.1 HEPES pH 7.5 and 20-25% ethylene glycol, VAPOR DIFFUSION, temperature 298 KK
1	VAPOR DIFFUSION	7.5	298	2.0-3.2 M ammonium formate, 0.1 HEPES pH 7.5 and 20-25% ethylene glycol, VAPOR DIFFUSION, temperature 298 KK