3QYA
Crystal structure of a red-emitter mutant of Lampyris turkestanicus luciferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-08 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 41 |
Unit cell lengths | 85.009, 85.009, 97.097 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.980 - 2.130 |
R-factor | 0.1672 |
Rwork | 0.165 |
R-free | 0.20760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ba3 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.559 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.370 | 2.190 | |
High resolution limit [Å] | 2.130 | 9.540 | 2.130 |
Rmerge | 0.133 | 0.021 | 0.644 |
Number of reflections | 38301 | 438 | 2768 |
<I/σ(I)> | 12.27 | 53.4 | 2.5 |
Completeness [%] | 99.7 | 97.1 | 97.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 4 % w/v PEG 8000, 14 % v/v Ethylenglycol, 100 mM Na-HEPES, pH 7.5, vapor diffusion, sitting drop, temperature 293K |