3QXF
Structure of the bacterial cellulose synthase subunit Z
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97874 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.817, 87.933, 91.754 |
| Unit cell angles | 69.96, 74.36, 78.22 |
Refinement procedure
| Resolution | 24.100 - 1.850 |
| R-factor | 0.13787 |
| Rwork | 0.136 |
| R-free | 0.17793 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.921 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 121857 |
| Completeness [%] | 99.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 30-40% PEG300, 0.1M sodium citrate/phosphate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
