3QWZ
Crystal structure of FAF1 UBX-p97N-domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-12 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.490, 59.938, 75.541 |
| Unit cell angles | 90.00, 114.83, 90.00 |
Refinement procedure
| Resolution | 19.330 - 2.000 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e32 |
| RMSD bond length | 0.042 |
| RMSD bond angle | 2.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | 0.219 |
| Number of reflections | 18769 | |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 96.5 | 91.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 295 | 30% PEG 3000, 0.1M sodium acetate, 1M lithium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
