3QWG
Crystal structure of EspRdelta10, C-terminal 10 amino acids deletion mutant of EspR transcription factor from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0097 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.460, 55.370, 76.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.860 - 1.992 |
| R-factor | 0.1852 |
| Rwork | 0.183 |
| R-free | 0.23560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qf3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.945 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CCP4 |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.860 | 2.110 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.104 | 0.387 |
| Number of reflections | 24707 | |
| <I/σ(I)> | 9.83 | 3.11 |
| Completeness [%] | 82.6 | |
| Redundancy | 3.4 | 3.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277.15 | 20% PEG 4000, 100mM Tris-HCl, 200 mM LiSO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
