3QW4
Structure of Leishmania donovani UMP synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 64.722, 64.722, 477.596 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.960 - 3.000 |
| R-factor | 0.23905 |
| Rwork | 0.236 |
| R-free | 0.30430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.080 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.100 |
| High resolution limit [Å] | 2.990 | 6.440 | 2.990 |
| Rmerge | 0.131 | 0.101 | 0.374 |
| Number of reflections | 21844 | ||
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 87.7 | 98.7 | 39.7 |
| Redundancy | 3.5 | 3.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 291 | 32 % Peg400, 250 mM NaCl 0.1 M Hepes, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
