3QW1

Crystal structure of the Zn-RIDC1 complex stabilized by BMH crosslinks

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-10
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	48.298, 61.843, 70.087
Unit cell angles	90.00, 102.22, 90.00

Refinement procedure

Resolution	47.200 - 2.700
R-factor	0.2898
Rwork	0.288
R-free	0.31790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.559
Data reduction software	DENZO
Data scaling software	SCALA (3.3.16)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	68.498	61.843	2.850
High resolution limit [Å]	2.700	8.540	2.700
Rmerge		0.026	0.104
Total number of observations		1296	5983
Number of reflections	11245
<I/σ(I)>	9.6	19.1	6.5
Completeness [%]	100.0	99.4	100
Redundancy	3.7	3.4	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	50 mM Bis-TRIS, 20% pentaerythritol ethoxylate, 200 mM ammonium sulfate, 2.12 mM zinc chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K